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ProteoLiposome Expression Kit

Product Name Catalog No. Qty Storage
ProteoLiposome Expression Kit CFS-TRI-PLE 1 -80Ž
Details

The ProteoLiposome Expression Kit from CellFree Sciences provides researchers with all necessary reagents to prepare proteoliposome complexes for their proteins of interest. The kit contains lyophilized liposomes prepared from soybean asolectin for easy reaction setup.
This kit had been designed to perform protein expression reactions on a 4 ml reaction scale using 6-well plates. However, the reaction scale can be reduced to a 1 ml reaction scale if only small amounts of protein are needed to conduct your experiments. The 1 ml translation reaction can be setup using 24-well plates. This manual provides the reagent volumes for both reaction sizes.
The reaction conditions used in this kit have been tested for the expression of various membrane proteins. A 1 ml reaction yielded for example for the G Protein-Coupled Taste Receptor T1R1 about 30 µg of protein in the purified proteoliposome fraction.

Figure 1: Illustration of proteoliposome preparation

Figure 1: Illustration of proteoliposome preparation

Figure 2: SDS-PAGE showing example data for the G Protein-Coupled Taste Receptor T1R1

Figure 2: SDS-PAGE showing example data for the G Protein-Coupled Taste Receptor T1R1

Kit Contents
Reagent name Qty Concentration Volume Box color Storage
WEPRO®7240 1 240 OD 1 ml White -80Ž
SUB-AMIX® SGC S1 1 40x 1.1 ml
SUB-AMIX® SGC S2 1 40x 1.1 ml
SUB-AMIX® SGC S3 1 40x 1.1 ml
SUB-AMIX® SGC S4 1 40x 1.1 ml
5x Transcription Buffer LM 1 5x 0.4 ml
NTP Mix 1 25 mM 0.2 ml
SP6 RNA Polymerase 1 80 U/μl 30 µl
RNase Inhibitor 1 80 U/μl 30 µl
Creatine Kinase 1 20 mg/ml 20 µl
Asolectin Liposome, lyophilized 6 (10 mg) Lyophilized Green -80Ž
Instruction Manual
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