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Why Choose Us - CellFree Sciences Co., Ltd.|The natural power of wheat driving science -wheat germ cell-free protein expression system-

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Why Choose Us

1 Protein expression rates/Solubility

Our system has higher expression rates and solubility than other protein expression systems.

Using our products and services may resolve issues with proteins that could not be expressed using other expression systems, or became insoluble after expression.

 

可溶性タンパク質として得られる割合

 

 

The highest yields can be obtained through our wheat germ cell free expression system.

目的タンパク質の収量比較

 

Comparison of yields per translation reaction using 240 µl synthesis reactions
*Target protein:Dihydrofolate reductase (DHFR)

2 Reproducibility/Scalability

The proteins obtained through our system have a high reproducibility,

meaning that the amounts expressed are more or less constant no matter how many times expression is carried out.

Once the yield of the target protein has been confirmed,

it is easy to carry out additional syntheses, upscale, and calculate costs, contributing to well-planned implementation.

 

Additional syntheses

追加合成

 

Upscaling

スケールアップ

 

*This image is for illustrative purposes only. Our products and services cannot be used to make cakes.

3 Ease of Operations

Target proteins can be obtained just by using a pipette to adjust the reagents,

and an incubator to control temperature. 

操作が容易

 

 

There are only four steps involved,

and there is none of the hassle and time required as for culturing cells.

 

操作が容易

 

 

Once the template DNA has been constructed, synthesis can be confirmed in just two days.

Target Proteins can be confirmed by SDS-PAGE/CBB staining.

4 No need for codon optimization

Since different species prefer to use different sets of codons (codon bias), there are times when changes are made to codons to ensure that they are the ones preferred by the host (codon optimization) when transferring a gene from one species to another species for protein expression.

 

A wheat germ cell-free protein expression system is less sensitive to codon bias, so there is no need for codon optimization, and the genes from the original species can be used as is.

The is no decrease in the amounts expressed, even for mRNA with a unique sequence.

 

操作が容易

 

 

*There are cases where codon optimization can increase synthetic yields.

5 Selectivity of synthesis conditions

As the RNase activity for our wheat germ extract is extremely low, the reaction can be divided into the 2 steps of the transcription reaction and translation reaction, allowing the pursuit of optimal conditions for the translation reaction.

 

ステップ1 転写反応 ステップ2 翻訳反応

 

 

Reaction conditions can be changed freely depending on the purpose, such as adding metal ions, detergent, lipid membranes (liposomes, nanodiscs, etc.), or stable isotope labeled amino acids at the time of the translation reaction, or co-expression using multiple mRNA.

 

合成条件の柔軟性

 

 

The reaction temperature (4~26℃) and reaction times can be changed freely depending on the needs of the target protein.

 

Generally, our recommended temperatures and reaction times are 20 hours at 15℃.

 

合成条件の柔軟性

6 Can be used around the world

The proteins that can be obtained by using our products and services do not fall within

the scope of the Act on the Conservation and Sustainable Use of Biological Diversity through Regulations on the Use of Living Modified Organisms.

It can contribute to international joint research projects and global strategies, as there are no obstacles such as export restrictions or the hassle and burden of procedures.

 

カルタヘナ法規制対象外

 

We also contribute to an international joint research project to develop vaccines to eradicate malaria.

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