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Jan 2014
GHIT
On November 8th, 2013, The Global Health Innovative Technology Fund (GHIT) announced the winners for their first Request for Proposals 2013-001 (RFP 2013-001) within which GHIT will fund several new global partnerships between Japanese and non-Japanese organizations for the development of new innovations for the patients in the developing world inflicted by infectious diseases.

CellFree Sciences is very grateful to have received in a partnership together with the Program for Appropriate Technology in Health's (PATH) Malaria Vaccine Initiative, based in Seattle, Washington, USA, and the Proteo-Science Center (PROS) at Ehime University based in Matsuyama, Ehime, Japan, US$ 600,000 from the GHIT Fund for the joint development of a novel screening method for finding new vaccine candidates for malaria.

Malaria is a mosquito-borne infectious disease caused by parasitic protozoans (an unicellular microorganism) that in severe cases and lack of treatment can lead to the death of infected patients. The disease is widespread in large parts of Africa, Asia, and the Americas, where the World Health Organization (WHO) reported for 2010 some 219 million documented cases of malaria. In that year only the disease may have killed about 660,000 people including a high number of children according to estimations of the WHO. Although malaria is one of the greatest health burdens of mankind that in particular damages the prospect of poor people and the developing world, as of today, it had not been possible to develop an effective vaccine against the disease.

One of the biggest problems in developing malaria vaccines is that only a very small number of potential vaccine components can be screened because of persisting problems in the preparation of proteins from parasitic protozoans. To overcome this problem scientists at Ehime University and CellFree Sciences have developed an innovative wheat germ cell-free protein synthesis technology that allows scientists to produce large numbers of malaria vaccine candidate proteins. Under the new initiative funded by GHIT, the PATH Malaria Vaccine Initiative will develop antibodies against parasite proteins prepared by Ehime University and CellFree Sciences to evaluate which of those proteins may induce the most effective immunity against the disease. This screening process will hopefully reveal new malaria vaccine candidates for further testing and evaluation in clinical trials.

"The GHIT Fund will play a decisive role in bringing Japanese know-how and investment to the global fight against infectious diseases." commented Dr. Matthias Harbers, the responsible project manager for this program at CellFree Sciences. "This project is a very good example for this as it is very much based on the prominent malaria vaccine research at the laboratory of Professor Tsuboi at Ehime University. In combination with the expertise at the PATH Malaria Vaccine Initiative, we believe this project will certainly make a significant contribution to the development of new malaria vaccines."

"We are excited to be part of this important project made possible by the funding of GHIT", added Satoshi Ozawa, CEO and President of CellFree Sciences. "With the efficiency of our protein expression system, I believe that we can make an important contribution to the development of new malaria vaccines in line with the objectives of the GHIT Fund. It is our sincere hope that this project will help to develop the vaccines needed for effective malaria eradication efforts."

LINKS FOR ADDITIONAL INFORMATION:
July 2012
Through collaboration between Mahidol University and CellFree Sciences Co., Ltd., Cell-Free Protein Synthesis Workshop was held at the Faculty of Tropical Medicine, Mahidol University, Thailand, from July 25 through July 28.
During the workshop, introductory lectures on the wheat germ cell-free system and its applications given, and more than 20 participants had hands-on experience in the wheat germ cell-free protein production.

Please visit the following URL for pictures of the workshop.
[http://www.tm.mahidol.ac.th/research/client/News.html]
Feb 2012
CFS launched Premium PLUS Expression Kit, a new two-in-one starter kit, which allows you to use one and the same kit with either plasmid DNA or PCR DNA templates. Based on the high performance wheat germ extract WEPRO®7240, the new kit enables a more than twofold increase in protein yield.
Jan 2012
During the PepTalk 2012, San Diego, CFS is presenting a poster in the Pipeline4, Choosing, Designing and Optimizing Hosts and Platforms Session, Jan 11-12, 2012. The title of the poster is “Wheat Germ Cell-free Protein Expression System: Yield improvement and expression of membrane proteins.”
June 2011
The 35th Steenbock Symposium ’Advances in Biomolecular NMR’ will be held June 26-28, 2011, at the University of Wisconsin, Madison. During the symposium, CellFree Sciences will be presenting its technology and products at the exhibition booth. The program of the symposium is available at
[http://www.biochem.wisc.edu/seminars/steenbock/symposium35].
May 2011
During the 2011 PEGS Meeting, Boston, CFS is presenting a poster in the Difficult to Express Proteins Session, May 9-10, 2011. The title of the poster is “Soluble Expression of Membrane Proteins in Wheat Germ Cell-Free Protein Expression System.”
March 2011
Please be advised that the recent earthquake and tsunami have not inflicted any damage on our offices, facilities, and families both in Matsuyama and in Yokohama, and that there has been no disruption in shipments of our products. The subsequent trouble at the nuclear power plant in Fukushima is unnerving, but Yokohama being 250 km south of Fukushima and Matsuyama farther to the southwest, we know the best we can do is staying well-informed and level-headed. Many of you have asked us about our health and safety, and we are grateful for your concern.
July 2010
CFS was cited recently in the following articles:

1. Making membrane proteins for structures: a trillion tiny tweaks,
Nature Methods 2010, 7(6), 429-434

2. FLEXIQuant: A Novel Tool for the Absolute Quantification of
Proteins, and the Simultaneous Identification and Quantification
of Potentially Modified Peptides, Journal of Proteome Research
2009, 8(5), 2201-2210

Nov 2008

On November 24, 2008, the National Institute of Advanced Industrial Science and Technology (AIST) announced the production of its “human protein factory” infrastructure for in vitro proteome research. The infrastructure includes a comprehensive collection of human Gateway entry clones of nearly 15,000 human genes, approximately 70% of the human genome, and their wheat germ cell-free protein expression data.

Synthesized in the wheat germ cell-free expression system, nearly all of those clones yielded proteins with soluble fractions. The research that led to the construction of the infrastructure was reported by Goshima and colleagues [1] in Nature Methods, December 2008, which was reviewed by Hartley and colleagues [2] in the same issue.

Information and expression data of the Gateway entry clones including SDS-PAGE patterns are available in the URL of Human Gene and Protein Database (HGPD) at http://hgpd.lifesciencedb.jp/ and information of the purchase of those clones is obtained by clicking Clone Inquiry in that URL.

[1] N. Goshima et al., Human protein factory for converting the transcriptome into an in vitro-expressed proteome, Nature Methods, 5, 1011-1017 (2008)

[2] J. L. Hartley et al., Proteome expression moves in vitro: resources and tools for harnessing the human proteome, Nature Methods, 5, 1001-1002 (2008)

July 2008
Malaria Vaccine Development - Malaria Protein Synthesis

“Research at Ehime University among 23 innovative technologies selected and supported by Japanese Government,” Ehime Shimbun, May 21, 2008

The Council for Science and Technology Policy chaired by Yasuo Fukuda, Prime minister of Japan, drafted a strategy for developing innovative technologies and selected 23 research projects as the global cutting-edge technologies. They will be funded from the next fiscal year onward by a new public fund established by the Council for accelerating the development of those innovative technologies. Among the recipients of new funding is the Malaria Vaccine Development Project at Ehime University using the Cell-Free Protein Expression System developed by Dr. Yaeta Endo of Ehime University.

The above strategy is aimed at enhancing the international competitiveness of industries, constructing healthy society, and strengthening the security of Japan and the rest of the world by supporting innovative technologies expected to have ripple effects on society. The selected technologies, one of which is regenerative therapy using iPS cells, encompass broad areas including information and communication technology, healthcare, and agriculture. Of the fiscal 2008 outlay of 1.4 trillion yen for science and technology promotion, 1% will be allocated to the innovative technology strategy.

In search of malaria vaccine candidates, Ehime University has started a joint research project with international collaborators including an Australian institute. In the heart of this search is the cell-free protein expression system, whose advent has made it possible to efficiently synthesize malaria parasite proteins beyond the limit of the commonly used E. coli system.

The Council for Science and Technology Policy states that the plant-based protein synthesis technology used in the Malaria Vaccine Development Project at Ehime University permits efficient production of a variety of malaria-related proteins for malaria vaccine development. By this statement, the Council expresses high expectation for the production of malaria vaccines in near future.
July 2008
Exhibition

Events: XXI Congress and General Assembly of the International Union of Crystallography
yIUCr2008 Osaka, Japan(http://www.iucr.org/iucr-top/index.html)z
Time and Dates: 9:00 - 17:00, August 24 - 27, 2008
Venue: Osaka International Convention Center (3F, Event Hall), Osaka, Japan
Exhibits: Wheat germ cell-free expression system, reagents, and kits. Introduction of a compact protein synthesizer for large-scale production for structural analysis
March 2007
Novel Analytical Method for Searching for Inhibitors of Cancer-Related Proteins

Joining forces with Taiho Pharmaceutical Co., Ltd., CellFree Sciences Co., Ltd. developed a novel analytical method for searching for inhibitors of cancer-related proteins. This followed Taiho's discovery of proteins including target proteins that play roles in cancer cell growth. We synthesized those proteins in our wheat germ cell-free expression system to develop a technique for high-throughput screening of compounds.

The new method permits (1) high-throughput search for inhibitors and (2) speedy identification of a specific protein that may be affected by an inhibitor and the specificity thereof. Its successful development is attributable to high-speed expression of active proteins using a desktop robot, Protemist DT, coupled with our newly developed high-speed protein modification and high-sensitivity assay technology.

Multiple units of Protemist DT have been in operation, besides Taiho, at Kyoto University, University of Tokyo, and other major research centers in Japan. Our services include development of tools for the functional analysis of proteins and protein-protein interaction. Two units of Protemist DT II, a new version of DT, have been added to the CESG of the University of Wisconsin, Madison.
October 2006
Cell free workshop at RIKEN Yokohama successfully completed. As a satellite conference of ICSG 2006, hands-on workshop at RIKEN Yokohama was held for 4 different courses from October 18th to 21st. The four workshops were completed with great success. Cell Free Sciences Co.Ltd joined the course 1,[Cell free protein expression course] as a co-sponsor providing premium expression kits to all the participants of course 1 to conduct protein expression experiment with wheat germ technologies. CFS also exhibited brand new auto-synthesizer called Protemist® DT-II. There were 28 scientists joined the course 1 from all over the world. We also introduced prototype synthesizer for large scale protein production in the course which caught high level of interest from the participants.
October 2006
We have entered into a strategic partnership today with Emerald BioSystems, Inc. (Emerald) to enhance each companyfs development, marketing, and sales activities in North America, Europe, and Japan. By working together, CFS and Emerald will make use of significant synergies in their respective product lines ranging from synthetic gene design software (Emeraldfs Gene Composer™) to sophisticated laboratory automation and reagents for cell-free protein production (CFSfs ENDEXT® reagents and Protemist® instruments) and to protein crystallization.
October 2006
Under the clear autumn sky, the 4th Protein Island Matsuyama (PIM) International Symposium 2006 was held at ANA Hotel Matsuyama on October 6, 2006. More than 200 researchers, engineers, and students listened to the presentations focused on potential applications of the cell-free protein synthesis technology. On Sunday, October 8, a seminar for the general public titled gFrom Ehime to the World - Challenges in Biosciencesh was held at the Matsuyama City Welfare Center. More than 350 people enjoyed the occasion. Please go to the following URL for more detail of the symposium:
http://www.pim-sympo.jp/2006/english/index.html
September 2006
R elated article on Wheat Germ Cell Free Protein Expression Workshop held at University of Wisconsin,Madison,WI.USA.
related article
September 2006
A unit of Protemist DT, CFSfs desktop protein synthesizer, has been installed at the laboratory of Professor Masayuki Murata of the University of Tokyo, Graduate School of Arts and Sciences. Being capable of producing and purifying proteins of interest overnight, the unit is to supply proteins to the screening system built by Professor Murata.
Designed for the functional analysis of proteins, Professor Muratafs screening system uses semi-intact cells in combination with visualization by an optical microscope. For this system, Protemist DT was selected as the best available protein synthesizer, with high credit given to its purification capability integrated into the fully automatic protein synthesis.
September 2006
A unique workshop for hands-on experience in wheat germ and other cell-free protein production systems will be held from 3 p.m. on October 18th to noon on October 21st at Yokohama City University and RIKEN Yokohama Institute. It is organized by Professor Yaeta Endo ( Ehime University ) and Project Director Shigeyuki Yokoyama ( RIKEN ) as one of the satellite workshops of the International Conference on Structural Genomics (ICSG) 2006.
CellFree Sciences, Co., Ltd. supports this workshop.
For more detail, please go to http://stone.gsc.riken.jp/icsg2006/index.html
August 2006
Wheat Germ Cell-Free Protein Production Workshop in University of Wisconsin ended successfully. Please see the following URL for hot news of the workshop. http://www.uwstructuralgenomics.org/cellfree_workshop.htm
July 2006
University of Wisconsin-Madison to hold Wheat Germ Cell-Free Protein Production Workshop

The Center for Eukaryotic Structural Genomics (CESG) and the Nuclear Magnetic Resonance Facility (NMRFAM) at the University of Wisconsin-Madison are holding the 2006 Wheat Germ Cell-Free Protein Production Workshop from July 30 through August 5, 2006. This unique workshop gives the participants hands-on experience in the wheat germ cell-free production of their own target proteins on a microgram and milligram scale. Seminars on related subjects are also held. Participants, representing major structural biology centers, academic institutes, and private companies, will come from US, Europe and Asia. The CellFree Sciences Co, Ltd. Japan, Promega Corporation, USA and Cambridge Isotope Laboratories, USA co-sponsor the workshop. The CESG at University of Wisconsin-Madison is one of the leading structural biology centers supported by the National Institute of General Medical Sciences (NIGMS) Protein Structure Initiative, ultimately aiming at making the three-dimensional atomic-level structures of most proteins easily obtainable from knowledge of their corresponding DNA sequences.

Prof. John Markley and Dr. Dmitriy Vinarov at the CESG in collaboration with Professor Yaeta Endo, Ehime University, and the CellFree Sciences, Co., Ltd have developed high throughput Wheat Germ Cell-Free Protein Production Platform for NMR, to breakthrough the bottleneck of high throughput labeled protein production. CESG has successfully determined 3-D structure of not less than 90 proteins, which have subsequently been registered at Protein Data Bank.
March 2006
We have renewed our Web Site.
January 2006
Pacific Northwest National Laboratory (PNNL), one of the nine national laboratories of the U.S. Department of Energy, employed the CFS automatic protein synthesizer GenDecoder1000. PNNL will use it to synthesize proteins required for Genomes to Life (GTL) program, whose focus is on the molecular machines of DOE-relevant microbes and their networking in living cells and microbial communities. GenDecoder1000 is capable of transcription and translation of 384 samples in an overnight campaign.
2004/09/06
Partnaring and Business Presentaion Session
2004/05/06
BIO2004 Annual International Convention
CellFree Sciences join the Japanese delegation in the world's largest bio-expo this year.
2004/04/09
The first exhibition at the 3rd INTERNATIONAL BIO EXPO JAPAN
2004/04/09
Operated Our Website.

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