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ProteoLiposome BD Expression Kit | CellFree Sciences

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Protein Expression Kits

Can be used to synthesize membrane proteins

Membrane protein expression Kit

ProteoLiposome BD Kit

ProteoLiposome BD Kit2

ProteoLiposome BD Kit

Comes as a set with reagents necessary for membrane protein synthesis.

Perfectly suited to large-scale production of membrane protein.

Features:

  • Membran protein has been synthesized using asolection liposome
  • Can be used for 6 reactions on bilayer-dialysis method 2.5 ml reaction scale.

  • Target proteins can be confirmed through SDS-PAGE/CBB staining

Recommended for:

  • Users who require yields of membrane protein several miligrams.

  • Users who want to perform antigen production using membrane protein antibodies.

  • Users who want to perform interaction analysis.

  • User who want to shorten the time taken by membrane protein synthesis.

 

*This kit does not come with a dialysis device included.

  A separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc.) is required.

 

The particle size of the liposomes in the solution has a peak distribution at about 150 nm based on scattered light intensity measurement, and the liposomes should be close to a monodispersed distribution after sonication. For more inforamtion, please refer to the instruction manual of this kit at p13.  

 

Flow of operations

ProteoLiposome BD Expression Kit img en

 

Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.

 

Specifications

Product Number

Product Name

Size

CFS-CPLE-BD

ProteoLiposome BD Kit

1 set

buy buton

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

WEPRO7240

240 OD

1 mL

1

SUB-AMIX SGC S1* 40x

7.6 mL

1

SUB-AMIX SGC S2*

40x

7.6 mL

1
SUB-AMIX SGC S3*
40x

7.6 mL

1
SUB-AMIX SGC S4*
40x

7.6 mL

1

Creatine Kinase

20 mg/mL

20 µL

1

NTPmix

25 mM

120 µL

1

5×Transcription Buffer LM

5x

240 µL

1

RNase Inhibitor

80K unit/mL

15 µL

1

SP6 RNA Polymerase

80K unit/mL

15 µL

1
Asolectin Liposome, lyophilized

10 mg

6
Storage Condition

 -80 ℃

 

* SUB-AMIX SGC S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each

Product-related Information

All our products are for research purpose only; not for use in diagnostic testing and use in human.

Contact us for more information and help on the use of our products.

 

Resources

Application Notes

 

Reference List

 

Related Products

WEPRO7240 Core Kit
WEPRO7240 Core Kit

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

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