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Advantages

Performance comparison among several expression systems
  ENDEXT®
(wheat germ)
Baculo
(in vivo)
E.coli
(in vivo)
E.coli
(in vitro)
Yeast CHO
Coverage* Excellent good poor poor good good
Folding
and
Activity
Good good poor poor poor good
Through-put Excellent poor poor good poor poor
Typical
process
time**
1 day 14 days 3-4 days 1 day 3-4 days 14 days
Amino
acids
labeling

Excellent

poor poor good poor poor
Glycosylation No insect type No No yeast type mammalian
type

* Coverage Kinds of proteins can be expressed; human, microbe, plant, virus

** Process time includes translation, collecting of cells, disruption of cells and recovery of soluble fraction.

Wheat germ cell-free system is suitable for structural determination especially for NMR analysis and functional analysis of proteins.

Protein production yield with ENDEXT® Technology
  per mL  WEPRO1240 per mL  WEPRO7240
Reaction method Bilayer Filter-and-Feed Bilayer Filter-and-Feed
GFP,DHFR, or GUS produced 1.6 mg 3 mg 3.2 mg 20 mg
Process comparison

Technology and paper

Wheat Germ Extract

  1. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes (2000)
  2. Sequence specificity and efficiency of protein N-terminal methionine elimination in wheat-embryo cell-free system (2007)

Expression Vector

  1. A set of ligation-independent in vitro translation vectors for eukaryotic protein production (2008)
  2. Selection of 5'-untranslated sequences that enhance initiation of translation in a cell-free protein synthesis system from wheat embryos (2005).

Small Scale, High-throughput Protein Production

  1. Genome-scale, biochemical annotation method based on the wheat germ cell-free protein synthesis system (2004)
  2. A cell-free protein synthesis system for high-throughput proteomics (2002)
  3. A bilayer cell-free protein synthesis system for high-throughput screening of gene products (2002)

Large Scale Protein Production (Antigen Production, Structural Determination)

  1. Novel protein fold discovered in the PabI family of restriction enzymes (2007)
  2. A wheat germ cell-free system is a novel way to screen protein folding and function (2003)

Robotic Protein Synthesizers

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