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Campaign : Special Offer until September 30, 2022: Synthesis Services for mono-biotinylated Proteins

Category: Announcements Created: Friday, 01 July 2022

Special Offer until September 30, 2022:

Synthesis Services for mono-biotinylated Proteins

Biotinylation of proteins is one of the most commonly use methods for working with labeled proteins. Based on the very strong interaction between biotin and Streptavidin, biotinylated proteins can be easily used in different assay formats. Our wheat germ cell-free system allows us to easily prepare mono-biotinylated proteins during translation reactions. Find out more on our approach and services for preparing mono-biotinylated proteins on our homepage:

https://www.cfsciences.com/eg/protein-biotinylation-services

Make use of our Special Offer on every order received until September 30, 2022, for using our services to prepare a mono-biotinylated protein. This offer includes:

  • Protein expression reaction on 6 ml bilayer format*,
  • Protein purification using His- or GST-tag**,
  • Protein QC by SDS-PAGE,
  • Confirmation of protein biotinylation by HABA Test***,
  • Protein stock provided in elution buffer plus glycerol****,
  • Negative control reaction without template can be provided.

*CFS can use different biotin concentrations in the translation reaction depending on how the biotinylated protein will be used.

**His- or GST-tag are normally placed on C terminus.

***Using Pierce™ Biotin Quantitation Kit to determine biotinylation level.

****Buffer exchange possible on customer request.

Service fee: JPY 240,000 (EURO 1,700, USD 2,000), shipping costs not included in service fee.

CFS will provide expression vector pEU-E01-bls-TEV-MCS free of charge to enable customers to prepare their own expression template. Otherwise, CFS can arrange for template preparation by gene synthesis at a fee of JPY 200,000 (EURO 1,400; USD 1,600).

Turnaround time: 4 weeks upon availability of expression vector; turnaround time for gene synthesis and vector preparation not included.

No foreseeing statements can be made about the expected protein yields.

Contact us for more information and ordering at: This email address is being protected from spambots. You need JavaScript enabled to view it..

 

CellFree Sciences Co., Ltd.

 

"CF-PPiD", a new protein-protein interaction (PPI) screening technology using human protein bead arrays, was reported in an academic paper.

Category: Press releases Created: Saturday, 25 June 2022

A new protein-protein interaction (PPI) screening technology "CF-PPiD" ,which applies "proximity-dependent biotin labeling technology" to the "20K Human Protein Bead Array" containing 20,000 human-derived proteins synthesized by the wheat cell-free synthesis system, has been reported as following academic paper. 

 

CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis Scientific Reports volume 12, Article number: 10592 (2022)

https://www.nature.com/articles/s41598-022-14872-w

 

Abstract

Protein–protein interaction (PPI) analysis is a key process to understand protein functions. Recently, we constructed a human protein array (20 K human protein beads array) consisting of 19,712 recombinant human proteins produced by a wheat cell-free protein production system. Here, we developed a cell-free protein array technology for proximity biotinylation-based PPI identification (CF-PPiD). The proximity biotinylation enzyme AirID-fused TP53 and -IκBα proteins each biotinylated specific interacting proteins on a 1536-well magnetic plate. In addition, AirID-fused cereblon was shown to have drug-inducible PPIs using CF-PPiD. Using the human protein beads array with AirID-IκBα, 132 proteins were biotinylated, and then selected clones showed these biological interactions in cells. Although ZBTB9 was not immunoprecipitated, it was highly biotinylated by AirID-IκBα, suggesting that this system detected weak interactions. These results indicated that CF-PPiD is useful for the biochemical identification of directly interacting proteins.

 

※ Now, we, Celfree Science Co. Ltd, are expanding our molecular interaction analysis service using "CF-PPiD" and looking forward to your use of our service.

 

 

New Year Campaign for Custom Synthesis Service is now on !!

Category: Announcements Created: Wednesday, 26 January 2022

We started New Year Campaign for Protein Syntehsis Service.

So, it is good time to try Wheat Germ Protein Systesis technology for your taget proteins, now.

 

Please access to "Campaign Page" on this Site, now.

 

CellFree Sciences Co., Ltd.

 

Season Greetings From CellFree Sciences

Category: Announcements Created: Tuesday, 21 December 2021

 

Season Greetings

From CellFree Sciences!

 

2022 Season Greeting

 

Our way of saying thank you.

 

With the year ending, we want to thank you for working together with CellFree Sciences and express our sincere wishes for the Seasons and a Happy, Healthy and Prosperous New Year!

 


This year had been complicated for all of us with life been very much dominated by the ongoing COVID-19 pandemic and the suffering it had broad to many people worldwide. But there is also hope in the times of the pandemic, which showed more than ever the importance of life sciences and the contribution we can make to bring the pandemic to an end. It fills us with pride that also our wheat germ cell-free expression system is used in the response to the COVID-19 pandemic to prepare viral proteins for research and the development and production of diagnostic products now available on the Japanese market. CellFree Sciences is committed to keep on supporting medical research and to work for better solutions in global health.

 


CellFree Sciences will start into 2022 with new assay service by using protein array for protein-protein interaction assay and "campaign for custom synthesis service" .

 


We all at CellFree Sciences are looking forward to being at your service again in 2022 supporting your important projects with our reagents and services. Please contact us and let us know about your needs and requirements. It is our commitment to work for our customers and partners to make your projects successful.

 


With all the very best wishes for you and yours for a better New Year 2022 from all of us at CellFree Sciences!

 

 

We will be @ The 44th Annual Meeting of the Molecular Biology Society of Japan

Category: Society & Exhibition Created: Thursday, 25 November 2021

CellFree Sciences will be at  the Exhibition of The 44th Annual Meeting of the Molecular Biology Society of Japanthe  at the PACIFICO Yokohama, Japan from December 1st to 3rd, 2021.

Please visit us at the following booth locations.

Booth No. A-77

We are looking forward to welcome you @ each booth  to provide you with more information on the use of our protein expression products, services and "Molecular Interaction Assay service with Human Beads Array".

 

We will be @ the Exhibition of The 68th Annual Meeting of the Japanese Society for Virology

Category: Society & Exhibition Created: Thursday, 11 November 2021

CellFree Sciences will be at the Exhibition of The 68th Annual Meeting of the Japanese Society for Virology at the Kobe International Conference Center, from November 16 to 18, 2021.

Please visit us at the following booth locations.

We are looking forward to welcome you @ our booth to provide you with more information on the use of our protein expression products and services for virus proteins.

Our research and development activities were introduced in the weekly Ehime Economic Report.

Category: Press releases Created: Thursday, 11 November 2021

The weekly Ehime Economic Report (November 8, 2021) No. 2273 introduced our efforts to develop "Targeted Proteolytic Drug (TPD)" in cooperation with Ehime University.

We will be @BioJapan2021

Category: Society & Exhibition Created: Monday, 27 September 2021

We will be @BioJapan2021 (https://jcd-expo.jp/en/)

CellFree Sciences will be at BioJapan 2021 at the PACIFICO Yokohama, Japan from October 13 to 15, 2021.

Please visit us at the following booth locations.

◇Booth No. and Name: 

C-67/CellFree Sciences with Ehime University (Protein Island Matsuyama)

D-3/CellFree Sciences with Japan Biological Informatics Consortium (JBIC)

 

We are looking forward to welcome you @ each booth and online partnering and to provide you with more information on the use of our protein expression products, services and molecular interaction assay service.

 

We will be at The 19th Protein Island Matsuyama International Symposium (PIM2021)

Category: Society & Exhibition Created: Monday, 06 September 2021

 CellFree Sciences will be at The 19th Protein Island Matsuyama International Symposium (PIM2021)The 19th Protein Island Matsuyama International Symposium (PIM2021)  at September 8, 2021.

【Date】Wednesday, September 8, 2021.

【Venue】Online

 Please visit us from Breakout session in Poster session

 We are looking forward to welcome you and to provide you with more information about " The introduction of Interaction assay by using Human Proein Beads Array" .

 

The movies introducing wheat cell-free protein synthesis technology and its applications is now available on the Protein Island Matsuyama (PIM) website.

Category: Press releases Created: Sunday, 01 August 2021

 PIM 01 1 e1552978512331

The movies introducing the wheat cell-free protein synthesis technology and its applications was created and released as one of the programs of Protein Island Matsuyama (PIM) 2020,

 

 

 

 

 


 

 


 

 

 

 

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