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Premium PLUS Expression Kit | CellFree Sciences

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Reagents & Kits


Can be used to synthesize all types of proteins

Premium PLUS Expression Kit

Recommended for first-time users and to synthesize protein on a small scale easily

Premium PLUS Expression Kit

Premium PLUS Expression Kit

Perfectly suited to confirm the expression of target proteins and synthesize protein on a small scale easily.

Features:

  • No need to prepare reagents
  • Reagents necessary for each reaction are already pre-packaged in tubes

  • Synthesize proteins with just a few pipetting steps

  • Target proteins can be confirmed through SDS-PAGE/CBB staining

  • Contains enough for 8 reactions on a 226 µl reaction scale

Recommended for:

  • Users who want to confirm the expression of target proteins easily using wheat germ cell-free expression system
  • Users who are having difficulties with protein expression using other protein expression system
  • Users who are having trouble because their target proteins are becoming insoluble

 

The reagents necessary for each reaction are pre-packaged into tubes, so there is no need to prepare any reagents.

Simply cut off the number of tubes per reaction you need, and you are ready to go.

 

Transcription reaction mixture (Transcription Premix LM)

transcription reaction mixture

 

Wheat germ extract (WEPRO9240)

wheat germ extract

 

Translation buffer (SUB-AMIX SGC)

translation buffer

 

Flow of operations

Premium PLUS Expression Kit img2 en

 

Specifications

Product Number

Product Name

Volume

CFS-EDX-PLUS

Premium PLUS Expression Kit

1 set

buy buton

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

pEU-E01-MCS

1 µg/µL

5 µL

1

pEU-E01-DHFR(Positive Control) 1 µg/µL 5 µL 1
PCR primer SPU 1 µM 100 µL 1
PCR primer deSP6E01 10 nM 100 µL 1
WEPRO9240*1 - 10 µL 8
SUB-AMIX SGC 1x 206 µL 8
Transcription Premix LM *2 1x 18 µL 8
Aluminum seal - - 2
Storage Condition

 -80 ℃

 

*1 : WEPRO9240 is based on WEPRO7240, with the addition of Creatine Kinase.

*2 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.

Product-related Information

All our products are for research purpose only; not for use in diagnostic testing and use in human.

Contact us for more information and help on the use of our products.

 

Resources

Application Notes

 

Reference List

 

Related Products

Protein Reseach Kit S3

Protein Research Kit S16

Standard type perfectly suited for easily small-scale synthesis of multiple samples.

Protein Reseach Kit G3

Protein Research Kit G16

Exhibits a higher level of GST-tagged fusion protein purity compared to Protein Research Kit S16.

Protein Reseach Kit H3
Protein Research Kit H16

Exhibits a higher level of His-tagged fusion protein purity compared to Protein Research Kit S16.

 WEPRO7240 Core Kit
WEPRO7240 Core Kit

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

WEPRO8240G Core Kit
WEPRO7240G Core Kit

Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO7240 Core Kit.

 WEPRO7240H Core Kit
WEPRO7240H Core Kit

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO7240 Core Kit.

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