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Can be used to synthesize all types of proteins His-tagged Protein Recommendation Protein Expression Kit WEPRO7240H Core Kit |
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WEPRO7240H Core Kit
Users can freely alter the reaction scale according to their goals and the quantity of protein required.
Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.
Improved removal performance against His-like proteins composed of wheat germ.
Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO7240 Core Kit.
Features:
- Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
- Target proteins can be confirmed through SDS-PAGE/CBB staining
Recommended for:
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Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.
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Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.
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Users who want to perform screening.
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Users who want to perform crystal structure analysis.
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Users who want to perform interaction analysis.
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Users who want to perform function analysis.
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Users who want to perform complex formation (co-expression).
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specifications
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Product Number |
Product Name |
Volume |
|---|---|---|
|
CFS-C7H |
WEPRO7240H Core Kit |
1 set |
Product Composition
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
WEPRO7240H |
240 OD |
1 mL |
1 |
| SUB-AMIX SGC S1* |
40x |
600 µL |
1 |
| SUB-AMIX SGC S2* |
40x | 600 µL | 1 |
| SUB-AMIX SGC S3* |
40x | 600 µL | 1 |
| SUB-AMIX SGC S4* |
40x | 600 µL | 1 |
|
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
| NTPmix |
25 mM |
120 µL |
1 |
|
5×Transcription Buffer LM |
5x |
240 µL |
1 |
|
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
|
SP6 RNA Polymerase |
80K unit/mL |
15 µL | 1 |
Storage Condition
-80 ℃
*SUB-AMIX SGC S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Resources
Related Products
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WEPRO7240 Core KitStandard type perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein. |
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WEPRO7240G Core KitExhibits a higher level of GST-tagged fusion protein purity compared to WEPRO7240 Core Kit. |
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Protein Research Kit H16Perfectly suited for easily small-scale synthesis of multiple samples. |
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Premium ONE Expression KitRecommended for first-time users and people who want to confirm the expression of target proteins right away. |
