MENU

WEPRO7240H Core Kit | CellFree Sciences

header jp

header en

Reagents & Kits

   New Design   

Can be used to synthesize all types of proteins

His-tagged Protein Recommendation

Protein Expression Kit

WEPRO7240H Core Kit

WEPRO7240H Core Kit 2

WEPRO7240H Core Kit

Users can freely alter the reaction scale according to their goals and the quantity of protein required. 

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

 

Improved removal performance against His-like proteins composed of wheat germ.

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO7240 Core Kit.

 

Features:

  • Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
  • Target proteins can be confirmed through SDS-PAGE/CBB staining

Recommended for:

  • Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.

  • Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.

  • Users who want to perform screening.

  • Users who want to perform crystal structure analysis.

  • Users who want to perform interaction analysis.

  • Users who want to perform function analysis.

  • Users who want to perform complex formation (co-expression).

 

Flow of operations

WEPRO7240 Expression Kit img en

 

Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.

 

Specifications

Product Number

Product Name

Volume

CFS-C7H

WEPRO7240H Core Kit

1 set


btn order support

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

WEPRO7240H

240 OD

1 mL

1

SUB-AMIX SGC S1*
40x

600 µL

1
SUB-AMIX SGC S2*
40x 600 µL 1
SUB-AMIX SGC S3*
40x 600 µL 1
SUB-AMIX SGC S4*
40x 600 µL 1

Creatine Kinase

20 mg/mL

20 µL

1
NTPmix

25 mM

120 µL

1

5×Transcription Buffer LM

5x

240 µL

1

RNase Inhibitor

80K unit/mL

15 µL

1

SP6 RNA Polymerase

80K unit/mL

15 µL 1
Storage Condition

 -80 ℃

 

*SUB-AMIX SGC S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each.

Resources

 

Application Notes

 

Reference List

 

Related Products

WEPRO7240 Core Kit
WEPRO7240 Core Kit

Standard type perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

WEPRO7240G Core Kit

WEPRO7240G Core Kit

Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO7240 Core Kit.

Protein Reseach Kit H3
Protein Research Kit H

Perfectly suited for easily small-scale synthesis of multiple samples.

Premium ONE Expression Kit 1
Premium ONE Expression Kit

Recommended for first-time users and people who want to confirm the expression of target proteins right away.

Copyright © CellFree Sciences Co.,Ltd. All Rights Reserved. [login]

We use cookies to ensure you get the best experience. By using our website you agree to our Cookie Policy.