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Split PCR Primers setThe Split Primers Set is a primer set designed to rapidly construct PCR-based expression templates optimized for the wheat germ cell-free protein synthesis system. |
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Split PCR Primers set
The Split PCR Primers Set is designed for the rapid construction of expression templates using the Split Primer PCR method.
This method uses a two-step PCR process to add an SP6 promoter, the E01 translational enhancer sequence, and a 3′ UTR to the target protein-coding sequence, enabling high-throughput preparation of linear DNA expression templates suitable for wheat germ cell-free protein synthesis.
The set also includes primers for constructing templates to produce His-tagged fusion proteins for purification.
Overview of the Split Primer PCR Method
Product lineup
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Product Number |
Product Name |
Content |
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CFS-SPCR-01 |
Split PCR Primers set |
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Safety Data Sheet (SDS)
Under preparation
Guide for Split Primer PCR method
Under preparation
Related Products
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WEPRO7240H Core Kit + Split PCR Primers set
The Split Primer PCR method enables rapid construction of expression templates, allowing the synthesis and His-tag purification of up to 96 different proteins at a reaction volume of 227 µL per protein. WEPRO7240H has been depleted of endogenous histidine-rich proteins derived from wheat germ, thereby improving purification performance. Compared with the WEPRO7240 Core Kit, it enables the production of His-tagged proteins with higher purity. |
