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膜タンパク質の合成

Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization

Fukumoto .A, et al. Communications Biology 2025, 8, Article number: 142

https://doi.org/10.1038/s42003-025-07564-4

The biophysical properties of TRIC-A and TRIC-B and their interactions with RyR2 

Jianshu Hu, et al., J Gen Physiol. 2023 155 (11): e202113070.

Evaluation of Cell-Free Synthesized Human Channel Proteins

for In Vitro Channel Research

Nishiguchi R. 1, et al. Membranes 2023, 13, 48.

MCAs in Arabidopsis are Ca2⁺-permeable mechanosensitive channels inherently sensitive to membrane tension

Kenjiro Yoshimura et. al., Nature Commun 2021 12, 6074.

Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay.

Takeda H. et al.: Sci Rep. 2015 Jun 10;5:11333.

PMID: 26061673 PMCID: PMC4462149

Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system.

Nozawa A. et al.: BMC Biotechnol. 2011 Apr 11;11:35.

PMID: 21481249 PMCID: PMC3090341

A cell-free method for expressing and reconstituting membrane proteins enables functional characterization of the plant receptor-like protein kinase FERONIA.

Minkoff B.B.: J Biol Chem. 2017 Apr 7;292(14):5932-5942.

PMID: 28235802 PMCID: PMC5392584

Modifications of wheat germ cell-free system for functional proteomics of plant membrane proteins.

Nozawa A., Tozawa Y.: Methods Mol Biol. 2014;1072:259-72.

PMID: 24136528

Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins.

Fogeron M.L. et al.: Methods Mol Biol. 2017;1635:91-108.

PMID: 28755365

Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes.

Suzuki Y. et al.: Front Pharmacol. 2018 Feb 6;9:38.

PMID: 29467651 PMCID: PMC5808195

Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5.

Hashimoto Y. et al.: Sci Rep. 2018 May 30;8(1):8383.

PMID: 29849184

Characterization of mitochondrial carrier proteins of malaria parasite Plasmodium falciparum based on in vitro translation and reconstitution.

Nozawa A. et al.: Parasitol Int. 2020 Jun 20;79:102160.

PMID: 32574727