|
Can be used to synthesize membrane proteins Membrane protein expression Kit ProteoLiposome BD Kit |
 |
ProteoLiposome BD Kit
Comes as a set with reagents necessary for membrane protein synthesis.
Perfectly suited to large-scale production of membrane protein.
Features:
- Membran protein has been synthesized using asolection liposome
-
Can be used for 6 reactions on bilayer-dialysis method 2.5 ml reaction scale.
- Target proteins can be confirmed through SDS-PAGE/CBB staining
Recommended for:
-
Users who require yields of membrane protein several miligrams.
-
Users who want to perform antigen production using membrane protein antibodies.
-
Users who want to perform interaction analysis.
-
User who want to shorten the time taken by membrane protein synthesis.
*This kit does not come with a dialysis device included.
A separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc.) is required.
The particle size of the liposomes in the solution has a peak distribution at about 150 nm based on scattered light intensity measurement, and the liposomes should be close to a monodispersed distribution after sonication. For more inforamtion, please refer to the instruction manual of this kit at p13.
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specifications
|
Product Number |
Product Name |
Size |
|---|---|---|
|
CFS-CPLE-BD |
ProteoLiposome BD Kit |
1 set |
Product Composition
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
WEPRO7240 |
240 OD |
1 mL |
1 |
| SUB-AMIX SGC S1* | 40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S2* |
40x |
7.6 mL |
1 |
| SUB-AMIX SGC S3* |
40x |
7.6 mL |
1 |
| SUB-AMIX SGC S4* |
40x |
7.6 mL |
1 |
|
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
|
NTPmix |
25 mM |
120 µL |
1 |
|
5×Transcription Buffer LM |
5x |
240 µL |
1 |
|
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
|
SP6 RNA Polymerase |
80K unit/mL |
15 µL |
1 |
| Asolectin Liposome, lyophilized |
－ |
10 mg |
6 |
Storage Condition
-80 ℃
* SUB-AMIX SGC S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Resources
Related Products
 |
WEPRO7240 Core KitPerfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein. |
