News
Now on Launching : NanoDisc BD Kit
We, CellFree Sciences Co., Ltd. launched "NanoDisc BD Kit", now !!
This new kit has following features ;
- WEPRO7240ND, a wheat germ extract optimized for Nanodisc synthesis
- With the Bi-Layer & Dialysis (BD) reaction, which has a proven track record in proteoliposome synthesis
- A new method for NanoDisc synthesis (patent pending) that co-expresses mRNA for MSP protein and mRNA for the target membrane protein.
This kit enables high-quality and high-efficiency synthesis of NanoDisc for membrane proteins that have been difficult to
express in the past, and is hoped to further advance research on functional and structural analysis.
=====================Product Information ==============================
-Catalogue Number : CFS-CNDE-BD
-Product Name NanoDisc BD Kit
-SIZE : 5 reaction with 2.0 mL reaction scales of Bi-layer & Dialysis (BD) reaction
-Stock Temperature : -80℃
-List Price : $4,000.- /kit
-Specification : Claudin 4 can be synthesized as NanoDisc with more than 100ug per
Bi-Layer & Dialysis reaction.
====================================================================
Please contact to us and to our sales agents in your region, now.
Campaign "Launching for NanoDisc BD Kit" !!
Campaign !!
“launching for NanoDisc BD Kit”
Now, we are offering a 25% off campaign for try to use NanoDisc BD Kit until the end of this December.
$4,000/kit to $3,000/ kit !!
And also, if you try Wheat germ Cell Free Protein expression system for the fist time…………
with the purchase of the NanoDisc BD kit, you will receive a free Premium PLUS Expression Kit to confirm of your target membrane proteins expression.
Please contact to us and to our sales agents in your region, now.
We will be @『2nd Targeted Protein Degradation Conference in Japan』
CellFree Sciences will be at『2nd Targeted Protein Degradation Conference in Japan』hosted by FIMECS Inc.at theShonan iPark, Japan from July 26-27th 2023.
In the session, TechnoPro Corporation, which is participating in the exhibition and collaborating with us, will introduce its contract service for assay starting from "Wheat Cell-Free Synthesis System".
Kinesin, a key biomolecular motor protein, can be successfully synthesized by wheat germ from CellFree Sciences
A group led by Assistant Professor Daisuke Inoue (Kyushu University) has successfully synthesised the biomolecular motor protein, kinesin, using wheat germ as a cell-free protein synthesis system and reported its activity. This novel finding is important because kinesin was not only synthesized in abundant amounts, but it was also shown to have a higher motor activity compared to that synthesized by E. coli. More importantly, not only WT kinesin was synthesized by wheat germ, but also several mutated and affinity-tagged kinesins were successfully synthesized as well. This is especially important because it shows that the wheat germ provided by CellFree Sciences is a reliable and fast method for producing wild type, mutated, and tagged proteins in a high-quality manner that will expedite further research.
Title: In vitro Synthesis and Design of Kinesin Biomolecular Motors by Cell-Free Protein Synthesis
Authors: Assistant Professor Daisuke Inoue (Faculty of Design, Kyushu University)
Keisuke Ohashi (Graduate School of Global Food Resources, Hokkaido University)
Associate Professor Taichi E. Takasuka (Research Faculty of Agriculture, Hokkaido University)
Professor Akira Kakugo (Graduate School of Science, Kyoto University)
Journal: ACS Synthetic Biology (part of the American Chemical Society Group)
URL: https://pubs.acs.org/doi/abs/10.1021/acssynbio.3c00235: DOI: 10.1021/acssynbio.3c00235
Image Credit: Daisuke Inoue, Keisuke Ohashi, Taichi E. Takasuka and Akira Kakugo.
We will be @ The 45th Annual Meeting of the Molecular Biology Society of Japan
CellFree Sciences will be at the Exhibition of The 45th Annual Meeting of the Molecular Biology Society of Japanat the PACIFICO Yokohama, Japan from Novenber 30th to December 2nd, 2022.
Please visit us at the following booth locations.
◇Booth No. 36
We are looking forward to welcome you @ each booth to provide you with more information on the use of our protein expression products, services and "Molecular Interaction Assay service with Human Beads Array".
We will be at The 20th Protein Island Matsuyama International Symposium (PIM2022)
CellFree Sciences will be at The 19th Protein Island Matsuyama International Symposium (PIM2022) .
【Date】Friday, September 16th, 2022.
【Venue】On site @ Nanka Memorial Hall of Johoku Campus, Ehime University
And please visit us in Poster session @ Alumni Hall.
We are looking forward to welcome you.
Sales & Marketing department
CellFree Sciences Co., Ltd
The movies introducing wheat cell-free protein synthesis technology and its applications is available on the Protein Island Matsuyama (PIM) website.
The movies introducing the wheat cell-free protein synthesis technology and its applications were created and released as one of the programs of Protein Island Matsuyama (PIM) .
Campaign : Special Offer until September 30, 2022: Synthesis Services for mono-biotinylated Proteins
Special Offer until September 30, 2022:
Synthesis Services for mono-biotinylated Proteins
Biotinylation of proteins is one of the most commonly use methods for working with labeled proteins. Based on the very strong interaction between biotin and Streptavidin, biotinylated proteins can be easily used in different assay formats. Our wheat germ cell-free system allows us to easily prepare mono-biotinylated proteins during translation reactions. Find out more on our approach and services for preparing mono-biotinylated proteins on our homepage:
https://www.cfsciences.com/eg/protein-biotinylation-services
Make use of our Special Offer on every order received until September 30, 2022, for using our services to prepare a mono-biotinylated protein. This offer includes:
- Protein expression reaction on 6 ml bilayer format*,
- Protein purification using His- or GST-tag**,
- Protein QC by SDS-PAGE,
- Confirmation of protein biotinylation by HABA Test***,
- Protein stock provided in elution buffer plus glycerol****,
- Negative control reaction without template can be provided.
*CFS can use different biotin concentrations in the translation reaction depending on how the biotinylated protein will be used.
**His- or GST-tag are normally placed on C terminus.
***Using Pierce™ Biotin Quantitation Kit to determine biotinylation level.
****Buffer exchange possible on customer request.
Service fee: JPY 240,000 (EURO 1,700, USD 2,000), shipping costs not included in service fee.
CFS will provide expression vector pEU-E01-bls-TEV-MCS free of charge to enable customers to prepare their own expression template. Otherwise, CFS can arrange for template preparation by gene synthesis at a fee of JPY 200,000 (EURO 1,400; USD 1,600).
Turnaround time: 4 weeks upon availability of expression vector; turnaround time for gene synthesis and vector preparation not included.
No foreseeing statements can be made about the expected protein yields.
Contact us for more information and ordering at: This email address is being protected from spambots. You need JavaScript enabled to view it..
CellFree Sciences Co., Ltd.
"CF-PPiD", a new protein-protein interaction (PPI) screening technology using human protein bead arrays, was reported in an academic paper.
A new protein-protein interaction (PPI) screening technology "CF-PPiD" ,which applies "proximity-dependent biotin labeling technology" to the "20K Human Protein Bead Array" containing 20,000 human-derived proteins synthesized by the wheat cell-free synthesis system, has been reported as following academic paper.
CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis Scientific Reports volume 12, Article number: 10592 (2022)
https://www.nature.com/articles/s41598-022-14872-w
Abstract
Protein–protein interaction (PPI) analysis is a key process to understand protein functions. Recently, we constructed a human protein array (20 K human protein beads array) consisting of 19,712 recombinant human proteins produced by a wheat cell-free protein production system. Here, we developed a cell-free protein array technology for proximity biotinylation-based PPI identification (CF-PPiD). The proximity biotinylation enzyme AirID-fused TP53 and -IκBα proteins each biotinylated specific interacting proteins on a 1536-well magnetic plate. In addition, AirID-fused cereblon was shown to have drug-inducible PPIs using CF-PPiD. Using the human protein beads array with AirID-IκBα, 132 proteins were biotinylated, and then selected clones showed these biological interactions in cells. Although ZBTB9 was not immunoprecipitated, it was highly biotinylated by AirID-IκBα, suggesting that this system detected weak interactions. These results indicated that CF-PPiD is useful for the biochemical identification of directly interacting proteins.
※ Now, ｗe, Celfree Science Co. Ltd, are expanding our molecular interaction analysis service using "CF-PPiD" and looking forward to your use of our service.
New Year Campaign for Custom Synthesis Service is now on !!
We started New Year Campaign for Protein Syntehsis Service.
So, it is good time to try Wheat Germ Protein Systesis technology for your taget proteins, now.
Please access to "Campaign Page" on this Site, now.
CellFree Sciences Co., Ltd.
