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Can be used to synthesize membrane proteins NanoDisc BD Kit |
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NanoDisc BD Kit
Comes as a set with reagents necessary for NanoDisc synthesis for membrane protein Perfectly suited to NanoDisc synthesis of membrane protein with good quality and yield
Features:
- NanoDisc has been synthesized using co-expression with Asolectine liposome, mRNA for MSP ( Membrane Scaffold Protein) synthesis and mRNA for target membrane protein
- Can be used for 5 reactions on 2ml of bilayer–dialysis method which has been established for proteoliposome sysnthesis
- Target proteins can be confirmed through SDS-PAGE/CBB staining.
Recommended for:
- Users who require yields of membrane proteins several hundred micrograms.
- Users who want to perform interaction analysis
- Users who want to perform structure analysis of membrane protein
This kit does not include dialysis device.
A separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc.) is required.
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specifications
|
Product Number |
Product Name |
Size |
|---|---|---|
|
CFS-CNDE-BD |
NanoDisc BD Kit |
5 rxn /1 set |
Product Composition
① NanoDisc BD Kit (white box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
Transcription Reaction |
|||
|
SP6 RNA Polymerase |
80K unit/mL |
15 µL |
1 |
|
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
|
NTPmix |
25 mM |
120 µL |
1 |
|
5×Transcription Buffer LM |
5x |
240 µL |
1 |
|
Translation reaction |
|||
|
WEPRO7240ND |
240OD |
1mL |
1 |
|
SUB-AMIX SGC S1** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S2** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S3** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S4** |
40x |
7.6 mL |
1 |
|
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
② ND Plasmid set (white box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
pEU-E01-MCS-TEV-SBP-C1 |
1 mg/ml |
10ul |
1 |
|
pEU-E01-MSP |
1 mg/ml |
85ul |
1 |
|
pEU-E01-CLDN4-SBP (Control) |
1 mg/ml |
10ul |
1 |
③ SA set (white box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
SUB-AMIX SGC S1** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S2** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S3** |
40x |
7.6 mL |
1 |
|
SUB-AMIX SGC S4** |
40x |
7.6 mL |
1 |
④ Asolectin liposome, lyopphilized (green box):
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
Asolectin Liposome, lyophilized |
- |
10 mg |
6 |
Storage Condition
-80 ℃
*WEPRO7240ND is based on WEPRO7240 which dedicated for NanoDisc synthesis
**SUB-AMIX SGC S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
