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Vector

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Dedicated expression vector that has been optimized for our wheat cell-free expression system

pEU Vector Series 

 

 

peu2

pEU Vector

pEU vector is an expression vector that has been highly optimized to maximize synthetic efficiency through a wheat germ cell-free protein expression system.

By introducing bacteriophage SP6 into the transcriptional promoter and a completely artificial E01 sequence selected from a randomized nucleic acid sequence pool into the translational enhancer, an mRNA with high translational activity can be produced, and protein synthesis efficiency can be maximized. In addition to the vector outlined above, our company also offers vectors with added His-tag, GST-tag and DYKDDDDK-tag that are suited to affinity purification. You can choose which one you need depending on the situation.

 

Exclusively for your first-time purchase of kits/reagents, this set will be included free of charge.

 

Please note: For your second and subsequent orders, purchase will be required.

Product lineup

Product Number

Product Name

Volume and Concentration

Size

Instruction manual

CFS-PEU-v1.0

pEU Vector set

 10 µL each  [0.3 mg/ml]*
 1 Set btn download

* pEU-E01-DHFR (positive control) is 1.0 mg/mL.


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Product Composition

Non-Tagged Vector

Product Name

Position of Tag

 

Vector Map

Sequence Data

pEU-E01-MCS

-

-

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His-Tagged Vector

Product Name

Position of Tag

Protease Recognition Site

Vector Map

Sequence Data

pEU-E01-His-TEV-MCS-N1

N-terminus

TEV

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btn download

pEU-E01-His-TEV-MCS-N2 N-terminus TEV

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pEU-E01-His-TEV-MCS-N3 N-terminus TEV

btn download

pEU-E01-MCS-TEV-His-C1 C-terminus TEV

btn download

btn download

pEU-E01-MCS-TEV-His-C2 C-terminus TEV

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pEU-E01-MCS-TEV-His-C3 C-terminus TEV

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GST-Tagged Vector

Product Name

Position of Tag

Protease Recognition Site

Vector Map

Sequence Data

pEU-E01-GST-PS-MCS-N1

N-terminus

PreScission

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btn download

pEU-E01-GST-PS-MCS-N2 N-terminus PreScission

btn download

pEU-E01-GST-PS-MCS-N3 N-terminus PreScission

btn download

pEU-E01-MCS-PS-GST-C1 C-terminus PreScission

btn download

btn download

pEU-E01-MCS-PS-GST-C2 C-terminus PreScission

btn download

DYKDDDDK-Tagged Vector

Product Name

Position of Tag

Protease Recognition Site

Vector Map

Sequence Data

pEU-E01-DYKDDDDK-MCS-N1

N-terminus

-

btn download

btn download

pEU-E01-DYKDDDDK-MCS-N2 N-terminus -

btn download

pEU-E01-DYKDDDDK-MCS-N3 N-terminus -

btn download

 

 

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