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Dedicated expression vector that has been optimized for our wheat cell-free expression system pEU Vector Series
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pEU Vector
pEU vector is an expression vector that has been highly optimized to maximize synthetic efficiency through a wheat germ cell-free protein expression system.
By introducing bacteriophage SP6 into the transcriptional promoter and a completely artificial E01 sequence selected from a randomized nucleic acid sequence pool into the translational enhancer, an mRNA with high translational activity can be produced, and protein synthesis efficiency can be maximized. In addition to the vector outlined above, our company also offers vectors with added His-tag, GST-tag and DYKDDDDK-tag that are suited to affinity purification. You can choose which one you need depending on the situation.
Exclusively for your first-time purchase of kits/reagents, this set will be included free of charge.
Please note: For your second and subsequent orders, purchase will be required.
Product lineup
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Product Number |
Product Name |
Volume and Concentration |
Size |
Instruction manual |
Safety Data Sheet |
|---|---|---|---|---|---|
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CFS-PEU-v1.0 |
pEU Vector set |
10 µL each [0.3 mg/ml]* |
1 Set |  |
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* pEU-E01-DHFR (positive control) is 1.0 mg/mL.
Product Composition
Non-Tagged Vector
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Product Name |
Position of Tag |
Vector Map |
Sequence Data | |
|---|---|---|---|---|
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pEU-E01-MCS |
- |
- |
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His-Tagged Vector
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Product Name |
Position of Tag |
Protease Recognition Site |
Vector Map |
Sequence Data |
|---|---|---|---|---|
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pEU-E01-His-TEV-MCS-N1 |
N-terminus |
TEV |
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| pEU-E01-His-TEV-MCS-N2 | N-terminus | TEV |
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| pEU-E01-His-TEV-MCS-N3 | N-terminus | TEV |
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| pEU-E01-MCS-TEV-His-C1 | C-terminus | TEV |
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| pEU-E01-MCS-TEV-His-C2 | C-terminus | TEV |
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| pEU-E01-MCS-TEV-His-C3 | C-terminus | TEV |
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GST-Tagged Vector
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Product Name |
Position of Tag |
Protease Recognition Site |
Vector Map |
Sequence Data |
|---|---|---|---|---|
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pEU-E01-GST-PS-MCS-N1 |
N-terminus |
PreScission |
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| pEU-E01-GST-PS-MCS-N2 | N-terminus | PreScission |
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| pEU-E01-GST-PS-MCS-N3 | N-terminus | PreScission |
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| pEU-E01-MCS-PS-GST-C1 | C-terminus | PreScission |
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| pEU-E01-MCS-PS-GST-C2 | C-terminus | PreScission |
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DYKDDDDK-Tagged Vector
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Product Name |
Position of Tag |
Protease Recognition Site |
Vector Map |
Sequence Data |
|---|---|---|---|---|
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pEU-E01-DYKDDDDK-MCS-N1 |
N-terminus |
- |
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| pEU-E01-DYKDDDDK-MCS-N2 | N-terminus | - |
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| pEU-E01-DYKDDDDK-MCS-N3 | N-terminus | - |
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Positive Control
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Product Name |
Position of Tag |
Vector Map |
Sequence Data | |
|---|---|---|---|---|
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pEU-E01-DHFR |
- |
- |
- |
- |
