MENU

Premium ONE Expression Kit | CellFree Sciences

header jp

header en

Reagents & Kits

Can be used to confirm the expression of target proteins in a short time

Premium ONE Expression Kit

Recommended for first-time users and people who want to confirm

the expression of target proteins right away

Premium ONE Expression Kit 3

 

Premium ONE Expression Kit

Coupled transcription and translation reaction format for rapid protein expression experiments enabling protein analysis by different detection methods such as SDS-PAGE, Western Blot Analysis, or working with labeled proteins.

Features:

  • The newly developed "WEPRO TT" allows for easy-to-use coupled transcription/translation reactions requiring just a few pipetting steps.
  • Can be expressed from linear DNA templates as well as using an expression vector encoding the Open Reading Frame (ORF) for the protein of interest (PCR protocol and sense primers provided).
  • Confirm expression of target proteins in the wheat germ cell-free system within hours starting directly from template DNA.

  • Kit provides limited amount fluorescently-labeled lysine (FluoroTect™ GreenLys tRNA*1) for easy detection of target proteins without use of radio isotopes.
  • Using the fluorescent lysine labeling method or Western Blotting, proteins may be analyzed already doing 2-hour expression reactions.
  • Proteins can also be detected by SDS-PAGE followed by Coomassie Brilliant Blue (CBB) staining commonly after 12 to 24-hour reactions depending on protein yields.

Recommended for:

  • Users who want to confirm the expression of target proteins in wheat germ cell-free expression system
  • Users who want to test expression of different protein fragments using a PCR method for rapid template preparation
  • Users who need small protein amounts for analysis and testing

Flow of operations

Premium ONE Expression kit en img2 2

 

Contains reagents for 24 reactions on a 55 µl reaction scale (FluoroTect™ GreenLys tRNA included for up to 8 reactions).

 

*1 Sourced and manufactured by Promega Corp.

*2 For fluorescent detection, FluoroTectTM GreenLys tRNA is required.

 

Specifications

Product Number

Product Name

Size

CFS-EDX-ONE

Premium ONE Expression Kit

24 reactions


btn order support

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

pEU-E01-MCS

1 µg/µL

5 µL

1

pEU-E01-DYKDDDDK-DHFR(Positive Control)

15 ng/µL

10 µL

1

SPU (PCR sense primer) 1 µM 100 µL 1
deSP6E01 (PCR sense primer) 10 nM 100 µL 1
WEPRO TTmix - 70 µL 1
SUB-AMIX TT - 1.3 mL 1
FluoroTectTM GreenLys tRNA - 4 µL 1
Storage Condition

 -80 ℃

 

Product-related Information

All our products are for research purpose only; not for use in diagnostic testing and use in human.

Contact us for more information and help on the use of our products.

 

Resources

 Application Notes

 

 Reference List

 

Related Products

Protein Reseach Kit S3

Protein Research Kit S16

Standard type perfectly suited for easily small-scale synthesis of multiple samples.

Protein Reseach Kit G3

Protein Research Kit G16

Exhibits a higher level of GST-tagged fusion protein purity compared to Protein Research Kit S16.

Protein Reseach Kit H3
Protein Research Kit H16

Exhibits a higher level of His-tagged fusion protein purity compared to Protein Research Kit S16.

WEPRO7240 Core Kit
WEPRO7240 Core Kit

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

WEPRO8240G Core Kit
WEPRO7240G Core Kit

Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO7240 Core Kit.

WEPRO7240H Core Kit
WEPRO7240H Core Kit

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO7240 Core Kit.

Copyright © CellFree Sciences Co.,Ltd. All Rights Reserved. [login]

We use cookies to ensure you get the best experience. By using our website you agree to our Cookie Policy.