MENU

Premium ONE Expression Kit | CellFree Sciences

header jp

header en

Reagents & Kits

Can be used to confirm the expression of target proteins in a short time

Premium ONE Expression Kit

Recommended for first-time users and people who want to confirm

the expression of target proteins right away

Premium ONE Expression Kit 3

 

Premium ONE Expression Kit

Coupled transcription and translation reaction format for rapid protein expression experiments enabling protein analysis by different detection methods such as SDS-PAGE, Western Blot Analysis, or working with labeled proteins.

Features:

  • The newly developed "WEPRO TT" allows for easy-to-use coupled transcription/translation reactions requiring just a few pipetting steps.
  • Confirm expression of target proteins in the wheat germ cell-free system within hours starting directly from template DNA.

  • Template DNA can be directly prepared by PCR avoiding any cloning of new expression vectors (PCR protocol and sense primers provided).

  • Using template preparation by a PCR method and a sensitive detection method, the experiment can be completed just within 1 day.

  • Kit provides limited amount fluorescently-labeled lysine (FluoroTect™ GreenLys tRNA*1) for easy detection of target proteins without use of radio isotopes.
  • Using the fluorescent lysine labeling method or Western Blotting, proteins may be analyzed already doing 2-hour expression reactions.
  • Proteins can also be detected by SDS-PAGE followed by Coomassie Brilliant Blue (CBB) staining commonly after 12 to 24-hour reactions depending on protein yields.

Recommended for:

  • Users who want to confirm the expression of target proteins in wheat germ cell-free expression system
  • Users who want to test expression of different protein fragments using a PCR method for rapid template preparation
  • Users who need small protein amounts for analysis and testing

Flow of operations

Premium ONE Expression kit en img1 2

 

Contains reagents for 24 reactions on a 55 µl reaction scale (FluoroTect™ GreenLys tRNA included for up to 8 reactions).

 

*1 Sourced and manufactured by Promega Corp.

*2 For fluorescent detection, FluoroTectTM GreenLys tRNA is required.

 

Specifications

Product Number

Product Name

Size

CFS-EDX-ONE

Premium ONE Expression Kit

24 reactions

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

pEU-E01-DYKDDDDK-DHFR(Positive Control)

1 µg/µL

5 µL

1

SPU (PCR sense primer) 1 µM 100 µL 1
deSP6E01 (PCR sense primer) 10 nM 100 µL 1
WEPRO TTmix - 70 µL 1
SUB-AMIX TT - 1.3 mL 1
FluoroTectTM GreenLys tRNA - 4 µL 1
Storage Condition

 -80 ℃

 

Resources

 

 Application Notes

 

Related Products

Protein Reseach Kit S3

Protein Research Kit S

Standard type perfectly suited for easily small-scale synthesis of multiple samples.

Protein Reseach Kit G3

Protein Research Kit G

Exhibits a higher level of GST-tagged fusion protein purity compared to Protein Research Kit S.

Protein Reseach Kit H3
Protein Research Kit H

Exhibits a higher level of His-tagged fusion protein purity compared to Protein Research Kit S.

 WEPRO7240 Core Kit
WEPRO7240 Core Kit

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

WEPRO8240G Core Kit
WEPRO7240G Core Kit

Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO7240 Core Kit.

 WEPRO7240H Core Kit
WEPRO7240H Core Kit

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO7240 Core Kit.

Copyright © CellFree Sciences Co.,Ltd. All Rights Reserved. [login]

We use cookies to ensure you get the best experience. By using our website you agree to our Cookie Policy.