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Can be used to make full-length isotope-labeled MS standards FLEXIQuant PLUS Expression KitRecommended for stable isotope protein labeling to conduct FLEXIQuant experiments for bottom-up proteomics |
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FLEXIQuant PLUS Expression Kit
This kit was designed to be user-friendly even for first-time users.
Our wheat germ cell-free expression system allows you to easily make full-length isotope-labeled MS standards.
Features:
- Incorporates stable 13C and 15N isotopes-labeled lysine and arginine residues suitable for working with trypsin digestion in bottom-up proteomics
- Very high labeling efficiency with incorporation rates of over 99%
- No need to purchase 13C and 15N stable isotope-labeled amino acids as they are already added to translation system
- Special expression and control vector provided to utilize FLEX-tag for absolute protein quantification by FLEXIQuant method
- All reagents included to directly prepare labeled proteins from DNA template
- Premixed reagents to synthesize proteins with just a few pipetting steps
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Translation buffer provided in special reaction cups for bilayer reactions
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Target protein expression can be confirmed through SDS-PAGE/CBB staining
- Kit contains reagents for 8 translation reactions on 226 µl reaction scale
Recommended for:
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Users who require full-length isotope-labeled MS standards easily using wheat germ cell-free expression system
All reagents are already aliquoted to provide the amounts needed per reaction.
This makes reaction setup easy and avoids unnecessary freeze-thawing of sensitive reagents.
Simply cut off the number of tubes needed for each experiment.
Flow of operations
Specifications
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Product Number |
Product Name |
Volume |
|---|---|---|
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CFS-FLEX-MS |
FLEXIQuant PLUS Expression Kit |
1 set |
Product Composition
Reference peptide
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Reagent Name |
Amount |
Content |
Quantity |
|---|---|---|---|
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FLEX-tag peptide |
1 nmol |
Dried powder |
8 |
Protein expression reagents and vectors
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Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
pEU-E01-MCS |
1 µg/µL |
5 µL |
1 |
| pEU-E01-His-FLEX-MCS-N1 | 1 µg/µL | 5 µL | 1 |
| pEU-E01-CDC27（Positive Control） | 1 µg/µL | 5 µL | 1 |
| WEPRO9240 for MS*1 | - | 10 µL | 8 |
| SUB-AMIX SGC for MS*2 | 1x | 206 µL | 8 |
| Transcription Premix LM *3 | 1x | 18 µL | 8 |
| Aluminum seal | - | - | 4 |
Storage Condition
Reference peptide: -20 ℃ under dry conditions
Protein expression reagents and vectors: -80 ℃
*1 : WEPRO9240 for MS is based on the WEPRO8240 with the addition of 13C and 15N-labeled lysine, arginine, and Creatine Kinase.
*2 : SUB-AMIX SGC for MS includes 18 types of non-labeled amino acids excluding lysine and arginine.
*3 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Resources
Related Products
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WEPRO8240 Core KitPerfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein. |
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WEPRO8240G Core KitExhibits a higher level of GST-tagged fusion protein purity compared to WEPRO8240 Core Kit. |
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WEPRO8240H Core KitExhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 Core Kit. |
