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FLEXIQuant PLUS Expression Kit | CellFree Sciences

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Reagents & Kits

Can be used to make full-length isotope-labeled MS standards

FLEXIQuant PLUS Expression Kit

Recommended for stable isotope protein labeling to conduct FLEXIQuant experiments for bottom-up proteomics

FLEXIQuant PLUS Expression Kit

FLEXIQuant PLUS Expression Kit

This kit was designed to be user-friendly even for first-time users.

Our wheat germ cell-free expression system allows you to easily make full-length isotope-labeled MS standards.

Features:

  • Incorporates stable 13C and 15isotopes-labeled lysine and arginine residues suitable for working with trypsin digestion in bottom-up proteomics
  • Very high labeling efficiency with incorporation rates of over 99%
  • No need to purchase 13C and 15stable isotope-labeled amino acids as they are already added to translation system
  • Special expression and control vector provided to utilize FLEX-tag for absolute protein quantification by FLEXIQuant method
  • All reagents included to directly prepare labeled proteins from DNA template
  • Premixed reagents to synthesize proteins with just a few pipetting steps
  • Translation buffer provided in special reaction cups for bilayer reactions

  • Target protein expression can be confirmed through SDS-PAGE/CBB staining

  • Kit contains reagents for 8 translation reactions on 226 µl reaction scale

Recommended for:

  • Users who require full-length isotope-labeled MS standards easily using wheat germ cell-free expression system

 

 

All reagents are already aliquoted to provide the amounts needed per reaction.

This makes reaction setup easy and avoids unnecessary freeze-thawing of sensitive reagents.

Simply cut off the number of tubes needed for each experiment.

 

Premium PLUS Expression Kit for MS img en

 

Flow of operations

Premium PLUS Expression Kit for MS img2 en

 

 

Specifications

Product Number

Product Name

Volume

CFS-FLEX-MS

FLEXIQuant PLUS Expression Kit

1 set


btn order support

 

 

Product Composition

Reference peptide

Reagent Name

Amount

Content

Quantity

FLEX-tag peptide                               

1 nmol

Dried powder

8

Protein expression reagents and vectors

Reagent Name

Concentration

Volume

Quantity

pEU-E01-MCS

1 µg/µL

5 µL

1

pEU-E01-His-FLEX-MCS-N1 1 µg/µL 5 µL 1
pEU-E01-CDC27(Positive Control) 1 µg/µL 5 µL 1
WEPRO9240 for MS*1 - 10 µL 8
SUB-AMIX SGC for MS*2 1x 206 µL 8
Transcription Premix LM *3 1x 18 µL 8
Aluminum seal - - 4
Storage Condition

Reference peptide: -20 ℃ under dry conditions

Protein expression reagents and vectors-80 ℃

 

*1 : WEPRO9240 for MS is based on the WEPRO8240 with the addition of 13 and 15N-labeled lysine, arginine, and Creatine Kinase.

*2 : SUB-AMIX SGC for MS includes 18 types of non-labeled amino acids excluding lysine and arginine.

*3 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.

Resources

 

 Application Notes

 

Related Products

WEPRO8240 Core Kit

WEPRO8240 Core Kit

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

WEPRO8240G Core Kit
WEPRO8240G Core Kit

Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO8240 Core Kit.

WEPRO8240H Core Kit
WEPRO8240H Core Kit

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 Core Kit.

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