Can be used to synthesize stable isotope labeled proteins GST-tagged Protein Recommendation Protein Expression Kit WEPRO8240G Core Kit |
WEPRO8240G Core Kit
Comes as a set with reagents necessary for stable isotope labeled proteins synthesis.
Synthesises by adjusting stable isotope labeled proteins, according to the customer’s goals.
Improved removal performance against GST-like proteins composed of wheat germ.
Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO8240 Core Kit.
* This kit does not come with isotope labeled amino acids included.
If the customer wants to prepare and adjust amino acids themselves, our order-made translation buffer can be used, requiring no separate amino acid adjustment.
Features:
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Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
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Target proteins can be confirmed through SDS-PAGE/CBB staining.
Recommended for:
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Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.
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Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.
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Users who want to perform screening.
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Users who want to perform structure analysis.
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Users who want to perform interaction analysis.
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Users who want to perform function analysis.
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specifications
Product Number |
Product Name |
Size |
---|---|---|
CFS-C8G |
WEPRO8240G Core Kit |
1 set |
Product Composition
Reagent Name |
Concentration |
Volume |
Quantity |
---|---|---|---|
WEPRO8240G |
240 OD |
1 mL |
1 |
SUB-AMIX SGC NA S1* | 40x | 600 µL | 1 |
SUB-AMIX SGC NA S2* | 40x | 600 µL | 1 |
SUB-AMIX SGC NA S3* | 40x | 600 µL | 1 |
SUB-AMIX SGC NA S4* | 40x | 600 µL | 1 |
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
NTPmix |
25 mM |
120 µL |
1 |
5×Transcription Buffer LM |
5x |
240 µL |
1 |
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
SP6 RNA Polymerase |
80K unit/mL | 15 µL | 1 |
Storage Condition
-80 ℃
*SUB-AMIX SGC NA S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Resources
Related Products
WEPRO8240 Core KitStandard type perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein. |
|
WEPRO8240H Core KitExhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 Core Kit. |
|
WEPRO8240G(15N) Core KitThe translation buffer contains stable isotope 15N labeled amino acids. Recommended for users who want to perform analysis of Heteronuclear single-quantum correlation spectroscopy (HSQC) spectrum. |
|
WEPRO8240G (13C, 15N) Core KitThe translation buffer contains stable isotope 13C and 15N labeled amino acids. Recommended for users who want to perform structure analysis using Nuclear Magnetic Resonance (NMR). |