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WEPRO8240G (13C/15N) Core Kit | CellFree Sciences

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Reagents & Kits

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Can be used to synthesize stable isotope 13C and 15labeled proteins

GST-tagged Protein Recommendation

Protein Expression Kit

WEPRO8240G (13C, 15N) Core Kit

WEPRO8240G 13C15N Core kit 2

WEPRO8240G (13C, 15N) Core Kit

Comes as a set with reagents necessary for stable isotope labeled protein synthesis.

The translation buffer contains stable isotope 13C and 15N labeled amino acids.

There is no need for customers to prepare an amino acid solution.

Improved removal performance against GST-like proteins composed of wheat germ.

Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO8240 (13C, 15N) Core Kit.

 

Features:

  • Synthesizes stable isotopes 13C and 15N labeled protein with over 99% efficiency.

  • There is no need for customers to prepare an amino acid solution.
  • Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.

  • Target proteins can be confirmed through SDS-PAGE/CBB staining.

Recommended for:

  • Users who require yields of stable isotopes 13C and 15N labeled protein ranging from a few hundred micrograms to several miligrams.

  • Users who want to perform structure analysis using Nuclear Magnetic Resonance (NMR).

 

Flow of operations

WEPRO7240 Expression Kit img en

 

Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.

 

Specifications

Product Number

Product Name

Volume

CFS-C8GCN

WEPRO8240G (13C, 15N) Core Kit

1 set


btn order support

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

WEPRO8240G

240 OD

1 mL

1

SUB-AMIX SGC (13C, 15N)  S1* 40x 600 µL 1
SUB-AMIX SGC (13C, 15N)  S2* 40x 600 µL 1
SUB-AMIX SGC (13C, 15N)  S3* 40x 600 µL 1
SUB-AMIX SGC (13C, 15N)  S4* 40x 600 µL 1

Creatine Kinase

20 mg/mL

20 µL

1
NTPmix

25 mM

120 µL

1

5×Transcription Buffer LM

5x

240 µL

1

RNase Inhibitor

80K unit/mL

15 µL

1

SP6 RNA Polymerase

80K unit/mL 15 µL 1
Storage Condition

 -80 ℃

 

*SUB-AMIX SGC(13C, 15N)S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each.

Resources

 

 Application Notes

 

 

Related Products

WEPRO8240 13C15N Core kit
WEPRO8240 (13C, 15N) Core Kit

Standard type perfectly suited for synthesizes as stable isotope 13C and 15N labeled protein.

WEPRO8240H 13C15N Core kit

WEPRO8240H (13C, 15N) Core Kit

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 (13C, 15N) Core Kit.

WEPRO8240G 15N Core kit
WEPRO8240G (15N) Core Kit

The translation buffer contains stable isotope 15N labeled amino acids.

Recommended for users who want to perform analysis of Heteronuclear single-quantum correlation spectroscopy (HSQC) spectrum.

WEPRO8240G Core Kit
WEPRO8240G Core Kit

The customer can prepare and adjust amino acids themselves.

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