Can be used to synthesize stable isotope 13C and 15N labeled proteins GST-tagged Protein Recommendation Protein Expression Kit WEPRO8240G (13C, 15N) Core Kit |  |
WEPRO8240G (13C, 15N) Core Kit
Comes as a set with reagents necessary for stable isotope labeled protein synthesis.
The translation buffer contains stable isotope 13C and 15N labeled amino acids.
There is no need for customers to prepare an amino acid solution.
Improved removal performance against GST-like proteins composed of wheat germ.
Exhibits a higher level of GST-tagged fusion protein purity compared to WEPRO8240 (13C, 15N) Core Kit.
Features:
Synthesizes stable isotopes 13C and 15N labeled protein with over 99% efficiency.
- There is no need for customers to prepare an amino acid solution.
Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
Target proteins can be confirmed through SDS-PAGE/CBB staining.
Recommended for:
Users who require yields of stable isotopes 13C and 15N labeled protein ranging from a few hundred micrograms to several miligrams.
Users who want to perform structure analysis using Nuclear Magnetic Resonance (NMR).
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specifications
Product Number | Product Name | Volume |
|---|---|---|
CFS-C8GCN | WEPRO8240G (13C, 15N) Core Kit | 1 set |
Product Composition
Reagent Name | Concentration | Volume | Quantity |
|---|---|---|---|
WEPRO8240G | 240 OD | 1 mL | 1 |
| SUB-AMIX SGC (13C, 15N) S1* | 40x | 600 µL | 1 |
| SUB-AMIX SGC (13C, 15N) S2* | 40x | 600 µL | 1 |
| SUB-AMIX SGC (13C, 15N) S3* | 40x | 600 µL | 1 |
| SUB-AMIX SGC (13C, 15N) S4* | 40x | 600 µL | 1 |
Creatine Kinase | 20 mg/mL | 20 µL | 1 |
| NTPmix | 25 mM | 120 µL | 1 |
5×Transcription Buffer LM | 5x | 240 µL | 1 |
RNase Inhibitor | 80K unit/mL | 15 µL | 1 |
SP6 RNA Polymerase | 80K unit/mL | 15 µL | 1 |
Storage Condition
-80 ℃
*SUB-AMIX SGC（13C, 15N）S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Resources
Related Products
 | WEPRO8240 (13C, 15N) Core KitStandard type perfectly suited for synthesizes as stable isotope 13C and 15N labeled protein. |
| WEPRO8240H (13C, 15N) Core KitExhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 (13C, 15N) Core Kit. |
 | WEPRO8240G (15N) Core KitThe translation buffer contains stable isotope 15N labeled amino acids. Recommended for users who want to perform analysis of Heteronuclear single-quantum correlation spectroscopy (HSQC) spectrum. |
 | WEPRO8240G Core KitThe customer can prepare and adjust amino acids themselves. |
