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Disulfide Bond Enhancer Enzyme Set | CellFree Sciences

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Intended for use in combination with WEPRO7240H Core Kit to make proteins having disulfide bonds

Disulfide Bond Enhancer Enzyme Set

Extension kit providing enzyme mix and reaction buffer

Disulfide Bond Enhancer Enzyme Set2 en

Disulfide Bond Enhancer Enzyme Set

This product does not contain any reagents to perform transcription and translation experiments.

 

It was designed to modify the reaction conditions for better formation of disulfide bonds when working with the WEPRO7240H Core Kit. Reaction conditions are modify by adding a human endoplasmic reticulum oxidoreductase (Ero1α) and a Protein disulfide isomerase (PDI) to the translation reaction while using a different buffer composition for bilayer formation. Learn more here on how to we support disulfide bond formation in our expression system.

 

Do not use the WEPRO7240 and WEPRO7240G Core kits as this will reduce protein yields. The GST-tag is unsuited when working with buffers containing glutathione as this will lead to a loss of disulfide bonds.

 

Confirm reaction conditions using the Disulfide Bond PLUS Expression Kit.

Features:

  • In combination with the WEPRO7240H Core Kit, this set allows to prepare proteins on different scales using the bilayer format

Recommended for:

  • Users who want to prepare proteins with disulfide bonds

Flow of operations 

Disulfide Bond Enhancer Enzyme Set img 2 en

 

Specifications

Product Number

Product Name

Size

CFS-DBEE

Disulfide Bond Enhancer Enzyme Set

1 Set 

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

SUB-AMIX SGC DTT-free S1

40×

600 µL

1

SUB-AMIX SGC DTT-free S2 40× 600 µL 1
SUB-AMIX SGC DTT-free S3 40× 600 µL 1
SUB-AMIX SGC DTT-free S4 40× 600 µL 1
PDI & Ero1α mix - 400 µL 1
Storage Condition

 -80 ℃

Product-related Information

All our products are for research purpose only; not for use in diagnostic testing and use in human.

Contact us for more information and help on the use of our products.

 

Applicasion Notes

Synthesis of tissue plasminogen activator (tPA) protease domain with retained activity

 

Synthesis of IgG Antibody Fab fragment

 

Related Products

WEPRO7240H Core Kit 2

WEPRO7240H Core Kit

Required for use of  Disulfide Bond Enhancer Enzyme Set to prepare proteins with disulfide bonds at different reaction scales.

Disulfide Bond PLUS Expression Kit 2 en

Disulfide Bond PLUS Expression Kit

Entry kit designed for ease use to test reaction conditions for disulfide bond formation.

This product supports 8 transcription and translation reactions working on 230 µl scale.

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