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Intended for use in combination with WEPRO7240H Core Kit to make proteins having disulfide bonds Disulfide Bond Enhancer Enzyme SetExtension kit providing enzyme mix and reaction buffer |
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Disulfide Bond Enhancer Enzyme Set
This product does not contain any reagents to perform transcription and translation experiments.
It was designed to modify the reaction conditions for better formation of disulfide bonds when working with the WEPRO7240H Core Kit. Reaction conditions are modify by adding a human endoplasmic reticulum oxidoreductase (Ero1α) and a Protein disulfide isomerase (PDI) to the translation reaction while using a different buffer composition for bilayer formation. Learn more here on how to we support disulfide bond formation in our expression system.
This kit does not contain reagents for transcription and translation reactions. WEPRO7240H Core Kit is required separately. Do not use the WEPRO7240 and WEPRO7240G Core kits as this will reduce protein yields. The GST-tag is unsuited when working with buffers containing glutathione as this will lead to a loss of disulfide bonds.
Confirm reaction conditions using the Disulfide Bond PLUS Expression Kit.
Features:
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In combination with the WEPRO7240H Core Kit, this set allows to prepare proteins on different scales using the bilayer format
Recommended for:
- Users who want to prepare proteins with disulfide bonds
Flow of operations
Specifications
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Product Number |
Product Name |
Size |
|---|---|---|
|
CFS-DBEE |
Disulfide Bond Enhancer Enzyme Set |
1 Set |
Product Composition
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Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
SUB-AMIX SGC DTT-free S1 |
40× |
600 µL |
1 |
| SUB-AMIX SGC DTT-free S2 | 40× | 600 µL | 1 |
| SUB-AMIX SGC DTT-free S3 | 40× | 600 µL | 1 |
| SUB-AMIX SGC DTT-free S4 | 40× | 600 µL | 1 |
| PDI & Ero1α mix | - | 400 µL | 1 |
Storage Condition
-80 ℃
Product-related Information
All our products are for research purpose only; not for use in diagnostic testing and use in human.
Contact us for more information and help on the use of our products.
Applicasion Notes
Synthesis of tissue plasminogen activator (tPA) protease domain with retained activity
Synthesis of IgG Antibody Fab fragment
Related Products
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WEPRO7240H Core KitRequired for use of Disulfide Bond Enhancer Enzyme Set to prepare proteins with disulfide bonds at different reaction scales. |
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Disulfide Bond PLUS Expression KitEntry kit designed for ease use to test reaction conditions for disulfide bond formation. This product supports 8 transcription and translation reactions working on 230 µl scale. |
